轉(zhuǎn)染小鼠胚胎成纖維細胞NIH-3T3 siRNA

轉(zhuǎn)染小鼠胚胎成纖維細胞NIH-3T3 siRNA （IF10.588）

zeta life轉(zhuǎn)染小鼠胚胎成纖維細胞NIH-3T3 siRNA (IF10.588)

zeta life轉(zhuǎn)染小鼠胚胎成纖維細胞NIH-3T3 IFIF10.588

zeta life高效率轉(zhuǎn)染NIH-3T3細胞 IFIF10.588

zeta life轉(zhuǎn)染NIH-3T3細胞-siRNA（IFIF10.588）


一、轉(zhuǎn)染小鼠胚胎成纖維細胞（NIH-3T3），轉(zhuǎn)染siRNA文章概述：

當(dāng)NIH/3T3細胞生長密度達到60%，在不含F(xiàn)BS培養(yǎng)基的六孔板中進行轉(zhuǎn)染。對于細胞轉(zhuǎn)染使用5μM pGPU6/GFP/Neo-shTRIM11,siALKBH5,miR-21a-5p mimic,miR-590–5p mimic, miR-361–3p mimic, miR-202–3p mimic和相應(yīng)對照，使用 Advanced DNA/RNA轉(zhuǎn)染試劑(#AD600025 Zeta life, USA) 對細胞轉(zhuǎn)染；在轉(zhuǎn)染24小時后分別對TRIM11或ALKBH5通過蛋白質(zhì)印跡或qPCR檢測敲低效率。

二、其它

1、NIH/3T3細胞培養(yǎng)條件：文章選擇Z7181FBS-500胎牛血清培養(yǎng)(# Zeta life, USA)
2、文章作者：河北醫(yī)科大學(xué)公共衛(wèi)生學(xué)院毒理學(xué)教研室、河北醫(yī)科大學(xué)環(huán)境與健康重點實驗室
3、標(biāo)題：FcγRIIB  The proteasome-dependent degradation of ALKBH5 regulates ECM deposition in PM2.5 exposure-induced pulmonary fibrosis of mice .(IF10.588)



 

三、轉(zhuǎn)染轉(zhuǎn)染NIH-3T3細胞原文

Cell transfection
Transfection was conducted when NIH/3T3 cells grown to 60% density in six-well plates with FBS absent medium. For cell transfection, 5 μM of pGPU6/GFP/Neo-shTRIM11, siALKBH5, miR-21a-5p mimic, miR-590–5p mimic, miR-361–3p mimic, miR-202–3p mimic and cor-responding control (Genepharma, Shanghai, China) was transfected into cells using Advanced DNA/RNA Transfection Reagent (Zeta life, USA) according to the supplier’s guidelines, respectively. TRIM11 or ALKBH5 knockdown efficiency was detected by western blotting or qPCR after 24 h post-transfection, respectively.

四、美國Zeta Life 公司與美國加利福尼亞大學(xué)舊金山校區(qū)聯(lián)合開發(fā)用于哺乳動物細胞、活體動物轉(zhuǎn)染的 Advanced DNA RNA 第三代多肽小分子轉(zhuǎn)染試劑，此技術(shù)成為新的全球蛋白功能、免疫細胞及干細胞治療、研發(fā)及生產(chǎn)的主要關(guān)鍵技術(shù)之一。

五、產(chǎn)品信息
產(chǎn)品名稱 貨號 規(guī)格
Advanced  DNA RNA  轉(zhuǎn)染試劑 AD600025 0.25ML
Advanced  DNA RNA  轉(zhuǎn)染試劑 AD600050 0.5ML
Advanced  DNA RNA  轉(zhuǎn)染試劑 AD600075 0.75ML
Advanced  DNA RNA  轉(zhuǎn)染試劑 AD600150 1.5ML