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LIPOPROTEIN DEFICIENT SERUM, HUMAN

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LIPOPROTEIN DEFICIENT SERUM, HUMAN

Catalog No:

YJ-1514

 

Lot No:

2017

Quantity:

50ml /vial

Concentration: >10.0 mg/ml (Protein)

Packaging:

Lipoprotein  Deficient  Serum  is  membrane  filtered  and  aseptically  packaged  in  a  solution

 

 

containing phosphate-buffered saline at pH 7.4.

 

 

 

Preparation:

AY   Lipoprotein Deficient Serum, LPDS, is prepared by ultracentrifugation, all of the lipoproteins

 

 

are removed and it is extensively dialyzed. The resultant LPDS is membrane filtered and packaged

 

 

aseptically. Each lot is evaluated in Human Skin Fibroblast or other cells in culture with our LDL and

 

 

[I125] LDL (or DiI-LDL and DiO-LDL) to determine the degree of up regulation of LDL receptors.

 

 

Typical lots give a 3 to 4 fold increase in [I125] LDL binding. We do not dilute the LPDS, typical

 

 

protein concentration for the human LPDS is >5.0mg/ml. .

 

 

Specifications:

0.45 micron filtered; Aseptically Filled; Cell Culture Tested.

 

Storage &

 

 

 

 

Stability:

This product is stable for 20 weeks after receipt when handled aseptically and stored at -18°C..

Special Note:

After prolonged storage, some precipitate may be observed.

This is normal for this product.  Clarify

 

 

out the aggregates by spinning in a microfuge for 2 minutes.

 

 

 

 

 

*Preparations of LPDS are fairly unstable; plan your experiments in advance and use fresh material.

 

FOR RESEARCH USE ONLY

 

 

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