LIPOPROTEIN DEFICIENT SERUM, HUMAN
Catalog No: |
YJ-1514 |
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Lot No: |
2017 |
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Quantity: |
50ml /vial |
Concentration: >10.0 mg/ml (Protein) |
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Packaging: |
Lipoprotein Deficient Serum is membrane filtered and aseptically packaged in a solution |
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containing phosphate-buffered saline at pH 7.4. |
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Preparation: |
AY Lipoprotein Deficient Serum, LPDS, is prepared by ultracentrifugation, all of the lipoproteins |
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are removed and it is extensively dialyzed. The resultant LPDS is membrane filtered and packaged |
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aseptically. Each lot is evaluated in Human Skin Fibroblast or other cells in culture with our LDL and |
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[I125] LDL (or DiI-LDL and DiO-LDL) to determine the degree of up regulation of LDL receptors. |
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Typical lots give a 3 to 4 fold increase in [I125] LDL binding. We do not dilute the LPDS, typical |
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protein concentration for the human LPDS is >5.0mg/ml. . |
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Specifications: |
0.45 micron filtered; Aseptically Filled; Cell Culture Tested. |
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Storage & |
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Stability: |
This product is stable for 20 weeks after receipt when handled aseptically and stored at -18°C.. |
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Special Note: |
After prolonged storage, some precipitate may be observed. |
This is normal for this product. Clarify |
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out the aggregates by spinning in a microfuge for 2 minutes. |
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*Preparations of LPDS are fairly unstable; plan your experiments in advance and use fresh material.
FOR RESEARCH USE ONLY